To get the isoelectric point of a protein(amino acids) or chemical molecule as output, you need to give input i.e dissociation constant of acid (pKa), dissociation constant of base(pKb) then click the calculate button. With the help of an isoelectric point calculator we get an accurate value of the isoelectric point of a protein. You just need to input the data in the input provision and hit the calculate button to avail the result.
Isoelectric Point Calculator: By using an isoelectric point calculator, we can find the isoelectric point for the given chemical molecule, if we have the value of dissociation constant of acid(pKa) and dissociation constant of base(pKb) we can find the isoelectric point of a chemical molecule. When we are calculating isoelectric points manually there are many chances of mistakes and also take more time for calculation.
Isoelectric point calculator helps us to get accurate value in less time. Here we will explain what is meant by isoelectric point, its formula, step by step procedure for how to use an isoelectric point calculator. Also we have solved and explained a few problems in a step by step process for better understanding. At the end of the article we can find frequently asked questions with solutions.
Isoelectric Point (pI, pH(I), IEP) is the pH value at which the particular molecule carries no net electrical charge or electrically neutral in statistical Mean. The concept is particularly important for zwitterionic molecules such as amino acids,peptides and proteins. The standard nomenclature to represent the isoelectric point is pH(I). Separation of proteins at the isoelectric point is called isoelectric focusing.
The formula for isoelectric point is Average sum of dissociation constant of an acid(pKa) and dissociation constant of base(pKb)
pI =(pKa +pKb)/2
Procedure to calculate isoelectric point is as follows
What is pI (Iso electric point) of Tyrosine?
Given that, dissociation constant of tyrosine are 2.2 and 9.11
pKa1 = 2.2
pKa2 = 9.11
Formula to find Isoelectric point pI = (pka1 +pka2)/2
Now substitute dissociation values in formula
pI = (2.2 +9.11)/2
pI = 5.66
Dissociation constant of acid is 5 and the dissociation constant of base is 4. Find the Isoelectric point of protein?
Given that, Dissociation constant of acid = 5
Dissociation constant of base = 4
Formula to find iso electric point pI = (pka1 +pka2)/2
Now substitute values in formula = (5+4)/2
Isoelectric point of protein is 4.5
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1. What is meant by an isoelectric point?
The isoelectric point (pI) is defined as the pH at which no net migration takes place in an electric field.
2. What is the formula for isoelectric point?
The formula for isoelectric point is the average sum of dissociation constant of an acid(ka) and dissociation constant of base(kb). pI =(pKa +pKb)/2
3. What is the isoelectric point of milk?
The isoelectric point of casein is 4.6. Since milk’s pH is 6.6,casein has a negative charge in milk
4. What is the use of isoelectric point?
The isoelectric point is significant in protein purification because it represents the pH where solubility is typically minimal.
5. What are the differences between pI and pH?
pH is the measure of acidity. It is the negative logarithm of concentration of hydrogen ions present in the solution, pI known as isoelectric point, this is the pH at which a molecule has a net neutral charge.
6. What is meant by the isoelectric point of amino acids?
The isoelectric point of amino acid is the point at which the amino acid has no net electric charge.